Rise in intracellular pH is concurrent with 'start' progression of Saccharomyces cerevisiae

Intracellular pH (pHi) was determined during arrest and recovery of temperature sensitive-cell division cycle mutants of Saccharomyces cerevisiae. In all mutants, pHi decreased during arrest; but when the mutants were released from arrest a rapid increase in pHi ensued in only cdc28- and cdc37-arrested cells. Both of these mutations cause arrest at 'start', the sole regulatory point in the S. cerevisiae cell cycle. In cells with cdc4 or cdc7 mutations, which arrest past start, pHi remained constant and exhibited a decrease, respectively, upon recovery of growth. The activity of plasma membrane ATPase decreased during the first 30 min of recovery of cdc28-arrested cells, concomitant with the rise in pHi. During the same period, there was no significant change in activity in cdc4-bearing cells, whereas an increase was observed for cdc7-bearing cells. Increase in pHi may be used as a specific signal by S. cerevisiae for start traversal and commitment to a new cycle

Novel Optimization to Reduce Power Drainage in Mobile Devices for Multicarrier-based Communication

With increasing adoption of multicarrier-based communications e.g. 3G and 4G, the users are significantly benefited with impressive data rate but at the cost of battery life of their mobile devices. We reviewed the existing techniques to find an open research gap in this regard. This paper presents a novel framework where an optimization is carried out with the objective function to maintain higher level of equilibrium between maximized data delivery and minimized transmit power. An analytical model considering multiple radio antennae in the mobile device is presented with constraint formulations of data quality and threshold power factor. The model outcome is evaluated with respect to amount of power being conserved as performance factor. The study was found to offer maximum energy conservation and the framework also suits well with existing communication system of mobile networks

(R1513) The Dynamical Study of Variable Mass Test Particle in Nonlinear Sense of Restricted 3-body Problem with Heterogeneous Primaries

The main idea of this paper is to study the non-linear stability property of the motion of the test particle which is moving under the influence of heterogeneous primaries having N-layers with different densities as well as varying its mass according to Jeans law. The system is also perturbed by the small perturbations in Coriolis as well as centrifugal forces. We evaluate the equations of motion of the test particle under the influence of the above said perturbations. From this system of equations of motion, we reveal analytically the locations of stationary points as well as the non-linear stability

Investigating ataxia in childhood

© Author(s) (or their employer(s)) 2019. No commercial re-use. See rights and permissions. Published by BMJ. Ataxia is a common presentation to an acute paediatric unit and it can often be difficult to determine the cause. It is important to distinguish between serious causes, for example, brain tumours and encephalitis, and more benign causes in order to guide investigations and treatment. In this review, we describe the different types of ataxia, the causes associated with them, the examination findings and what investigations to perform in order to make a diagnosis

Fabrication of Electrospun Composite Nanofibers and Their Application in Early Detection of Femtogram C- reactive protein (CRP)

The methodology for developing a protein biosensor has been presented and it focuses on the use of carbon nanotubes and polymer nanofibers based nano composites that have been electrospun in the form of “nanowebs”. These nanowebs have been surface modified to function as sensors for detecting C-reactive protein(CRP). Detection sensitivity of CRP was achieved in the femtogram/mL regime from phosphate buffered saline solutions and in human serum. The designed biosensor is three orders more sensitive than ELISA. Sensitivity enhancement was achieved by tuning the charge transduction at the solid /liquid interface on the nanoweb matrix

(R1985) Study the Effect of Modified Newtonian Force on the Restricted 3-body Configuration in Non-Linear Sense

This paper aims to investigate the non-linear stability of the triangular libration point in the restricted three-body problem (R3BP). The model, we use for our problem consists of a primary body as a heterogeneous spheroid with N-layers having different densities of each layer and a secondary body as a point mass that is producing the modified Newtonian Potential. We determine the equation of motion of the smallest body which is under the influence of the above-mentioned perturbations and also influenced by Coriolis as well as Centrifugal forces and then evaluated the Lagrangian for the evaluated system of equations. Afterwards, we write the first and second-order normalization of the Hamiltonian of the problem. By implying KAM theorem and the techniques used by Bhatnagar and Hallan, we discuss the non-linear stability analytically

A clinicoepidemiological study of geriatric dermatoses

Skin diseases are a common and inevitable consequence of ageing. Moreover, the clinical presentation is not as classical as they do in the younger population. A lifetime of solar exposure, along with intrinsic changes in the dermal structures, predisposes to a variety of skin diseases. The aim: to study the spectrum of various geriatric dermatoses among our patient population at the Department of Dermatology, Venereology, and Leprosy at Kamineni Academy of Medical Sciences and Research Centre. Materials and methods: in this study, a total of 200 patients aged 60 years and above attending the DVL OPD of Kamineni Academy of Medical Sciences and Research Centre were included. Results: maximum number of patients in this study belonged to 60-65 years (60 %), Male to female ratio was 1.86:1. Most of the males had agriculture work, and most of the females were housewives. Diabetes mellitus was the commonest associated systemic disease seen in 68 cases (34 %), and generalised pruritus was the commonest symptom seen in 64 (32 %) cases, of which 42 cases (65.6 %) were associated with xerosis. Pathological skin disorders and eczematous conditions were seen in 56 out of 200 cases. Of this, asteatotic eczema was the common finding among the eczematous conditions seen in 14 cases (7 %). Psoriasis was seen in 32 (16 %) and lichen planus in 10 cases (5 %). Infectious diseases were seen in 78 cases (39 %). Of these, fungal infections were common, seen in 28 cases (14 %). The benign tumour was seborrheic keratosis in this study, seen in 61 cases (30.5 %); among the malignant tumours, 4 cases (2 %) of basal cell carcinoma and 2 cases (1 %) of squamous cell carcinoma were seen. Among 16 cases of bullous disorders, bullous pemphigoid was seen in 12 (6 %) cases. Among 22 cases of psychocutaneous disorders, delusional parasitosis was seen in 10 cases (5 %), and perforating folliculitis in 15 cases (7.5 %). Loss of luster was the commonest nail change seen in 182 cases (91 %), followed by nail plate thickening in 54 cases (27 %). Greying of the hair was seen in all cases. Out of 70 females, diffuse hair loss was seen in 58 cases (82.9 %), and out of 130 males, androgenetic alopecia was seen in 72 cases (55.4 %). Conclusion: skin diseases cause considerable morbidity in the elderly, particularly if associated with other comorbid conditions. Health education on proper skin care, avoidance of irritants and self-medication etc., would help to reduce the incidence of common dermatoses

Preliminary phytochemical screening and Evaluation of hepatoprotective activity of ethanolic extract of whole plant of Evolvulus alsinoides using CCl4 induced model in experimental animals

Present study was conducted to evaluate the preliminary phytochemical screening and hepatoprotective activity of whole plant of Evolvulus alsinoides. The whole plant was defatted with petroleum ether and then extracted with 90 % ethanol. The phytochemical screening was done for ethanol extract using standard procedures. Acute toxicity was done using OECD 423 guidelines and the extract was found to be practically non-toxic upto a dose of 1500mg/kg b.w. when given orally. Hepatoprotective activity was evaluated using Carbon tetra chloride induced model in rats. In CCl4 induced mothod of 90% Ethanolic extract of whole plant of Evolvulus alsinoides (75 & 150mg/kg b.w.) produced significant dose dependent hepatoprotective activity. Histopathological studies could be carried out to assess the degree of damage. The hepatoprotective effect of the aforesaid extract was substantiated by pentobarbital sleeping time experiment in mice. The effect of the extracts at 150 mg/kg was compared to that of the reference drug, Silymarin (50 mg/kg). In vitro antioxidant studies were conducted to confirm the antioxidant mechanism involved in their hepatoprotective activity in CCl4-induced in rats. The presence of flavonoids could be responsible for hepatoprotective activity

Role of Work Life Balance on Psychological Wellbeing of the Teaching Professionals among Bangalore Institutions

Organisation today emphasize balance as paramount interest affected by the personality aspects, family aspects, support scenario, organizational culture and the existence of policies and their usage at the organizational level. Work Life balance as an issue has been explored from both an organizational and individual perspective. The current study aims at understanding. Role of work life balance on psychological wellbeing of the teaching professionals. Both the group were again administered with Psychological wellbeing by Dr.Sudha Bhogle and Dr.Indira to measure their level of Psychological wellbeing and the role of work life balance on occupational stress. A sample of 180 teachers 90 male and 90 female from various colleges of Bengaluru were administered. Directional hypothesis was formulated and the obtained results were statistically analyzed using independent samples‘t’ tests. The obtained’ scores on Psychological wellbeing indicated that there is a significant difference between both the groups, based on the mean scores we can infer that teachers with high work life balance have demonstrated little higher psychological wellbeing compared to teachers with low work life balance index

Detection of Cardiovascular CRP Protein Biomarker Using a Novel Nanofibrous Substrate

It is known that different diseases have characteristic biomarkers that are secreted very early on, even before the symptoms have developed. Before any kind of therapeutic approach can be used, it is necessary that such biomarkers be detected at a minimum concentration in the bodily fluids. Here, we report the fabrication of an interdigitated sensing device integrated with polyvinyl alcohol (PVA) nanofibers and carbon nanotubes (CNT) for the detection of an inflammatory biomarker, C-reactive protein (CRP). The limit of detection (LOD) was achieved in a range of 100 ng mL−1 and 1 fg mL−1 in both phosphate buffered saline (PBS) and human serum (hs). Furthermore, a significant change in the electrochemical impedance from 45% to 70% (hs) and 38% to 60% (PBS) over the loading range of CRP was achieved. The finite element analysis indicates that a non-redox charge transduction at the solid/liquid interface on the electrode surface is responsible for the enhanced sensitivity. Furthermore, the fabricated biosensor consists of a large electro-active surface area, along with better charge transfer characteristics that enabled improved specific binding with CRP. This was determined both experimentally and from the simulated electrochemical impedance of the PVA nanofiber patterned gold electrode.https://doi.org/10.3390/bios1006007